pathogens & biofilm (15)

29 Jul 2019

Testing the Water

Neelam R. Redekar and Jennifer L. Parke (Oregon State University)

We describe a simple method that growers can use to test their irrigation water for Phytophthora. Rhododendron leaves or pear fruits are used to bait Phytophthora from the water source. If brown spots develop on the baits, detection of Phytophthora may be confirmed by using an inexpensive diagnostic test kit. We provide links to short videos on how to bait and how to use the test kits.

Read the article here

25 Jun 2019

Diversity of Phytophthora, Pythium, and Phytopythium Species in Recycled Irrigation Water in a Container Nursery

Neelam R. Redekar, Joyce L. Eberhart, and Jennifer L. Parke (Oregon State University)

Plant nurseries may irrigate with surface or recycled water that is contaminated with plant pathogens. At an Oregon nursery we filtered or baited irrigation water, followed by next generation DNA sequencing, to identify multiple oomycete pathogens occurring together. Baits were more effective than filters at capturing pathogenic species. Pathogens were more abundant in recycled water than in the source water, but chlorination was effective at killing them. Pythium and Phytopythium species were prominent in summer, whereas Phytophthora species were observed year-round. Growers should regularly test irrigation water for pathogens and disinfest water before use in irrigation.

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26 Apr 2018

Test Your Irrigation Water for Phytophthora

Redekar, N.R. and Parke, J.L. (Oregon State University)

Phytophthora is a plant pathogen that can infect a wide variety of nursery plant species, and it spreads in irrigation water. In this article, we will describe how you can test your irrigation water for Phytophthora using baits. We present a case study of a large nursery where baiting was used to test the efficacy of their water treatment. Although this nursery recycles 90% of their irrigation water, we showed that they were successful at controlling Phytophthora contamination with their chlorination treatments.

Haga clic aquí para leer el artículo en español.

American Nurseryman Redekar and Parke 2018 (657 KB)

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Description of research activities

A national team of scientists is working to encourage use of alternative water resources by the nation’s billion-dollar nursery and floriculture industry has been awarded funds for the first year of an $8.7 million, five year US Department of Agriculture – National Institute of Food and Agriculture –Specialty Crop Research Initiative competitive grant.

The team will develop and apply systems-based solutions to assist grower decision making by providing science-based information to increase use of recycled water.  This award from the NIFA’s Specialty Crop Research Initiative is managed by Project Director Sarah White of Clemson University.  She leads a group of 21 scientists from nine U.S. institutions.

Entitled “Clean WateR3 - Reduce, Remediate, Recycle – Enhancing Alternative Water Resources Availability and Use to Increase Profitability in Specialty Crops”, the Clean WateR3 team will assist the grower decision-making process by providing science-based information on nutrient, pathogen, and pesticide fate in recycled water both before and after treatment, average cost and return-on investment of technologies examined, and model-derived, site specific recommendations for water management.  The trans-disciplinary Clean WateR3 team will develop these systems-based solutions by integrating sociological, economic, modeling, and biological data into a user-friendly decision-support system intended to inform and direct our stakeholders’ water management decision-making process.

The Clean WateR3 grant team is working with a stakeholder group of greenhouse and nursery growers throughout the United States.

For example, at the University of Florida graduate student George Grant is collecting data on removal of paclobutrazol, a highly persistent plant growth regulator chemical, from recirculated water using granular activated carbon (GAC) filters. This is being done in both research greenhouses and in a commercial site. The GAC filters can remove more than 90% of chemical residues, and are proving to be a cost-effective treatment method.